Load 50 µL of the 2 ng/µL rhPLAT into a black well plate, and start the reaction by adding 50 µL of 200 µM Substrate.Dilute Substrate to 200 µM in Assay Buffer.Dilute rhPLAT to 2 ng/µL in Assay Buffer.Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent.F16 Black Maxisorp Plate (Nunc, Catalog # 475515).Fluorogenic Peptide Substrate Z-Gly-Gly-Arg-AMC (Bachem, Catalog # I-1140), 10 mM stock in DMSO.Recombinant Human t‑Plasminogen Activator/tPA (rhPLAT) (Catalog # 7449-SE).
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |